Cancers and Gut Microbiome Biofilm
Cancer is cellular, so aren’t microbes involved?
An estimated 30% of all cancers are linked to microbial agents. Microbes are intimately involved in many cancers. They influence the risk for developing cancer, immune responses that affect both cancer and tumor control, disease progression, and response to treatment. Bio-sacal, as a prebiotic, focuses on the microbiome, which is something we’ve been doing since 2011.
The number of human cells is approximately 35 to 40 trillion (approximately 23,000 genes per cell) for adults, and the number of microorganisms in the body is approximately 38 to 42 trillion (3 million genes). Humans have 8 to 17 carbohydrate-decomposing enzymes, but intestinal microorganisms have approximately 15,000 to 20,000 carbohydrate-decomposing enzymes. Most of these microorganisms live in the intestines, and there are approximately 800 to 1200 species, depending on eating habits, climate, and race.
We know through many microorganism studies that these microorganisms have a good or bad effect on the immune system depending on the chemicals they secrete, and 70 to 80% of immune function comes from the large intestine. Therefore, managing intestinal microorganisms through diet is most important.
Most of these exist in the large intestine, but they are distributed to all organs from the skin and mouth. They exist not only in acute and chronic inflammation and tumors but even in brain cells. P. gingivalis, which is found in the brains of dementia patients, is shocking us. Therefore, the production of abundant short-chain fatty acids (SCFA) through colonic microbes is very important for the formation of healthy immunogens (IgN). In other words, if the SCFA level is low, the probability of inducing various cancers, ulcers, dementia, autism, depression, allergies, rare diseases, and mental illness increases. In particular, there seem to be few scholars who oppose the claim that colon cancer and rectal cancer are largely caused by colonic bacteria.
Microbial involvement in leading causes of death in the US (Centers for Disease Control 2017)
Asterisks (*) denote the evaluation of the strength of microbial involvement:
- Heart disease: 633,842 *** (an estimated 30% of all cancers are linked to microbial agents)
- Cancer: 595,930 ** (an estimated 20% of all cancers are linked to microbial agents)
- Chronic lower respiratory disease: 155,041 **
- Accidents: 146,571
- Stroke (cerebrovascular disease): 140,323 **
- Alzheimer’s disease: 110,561 ***
- Diabetes: 79,535 ***
- Influenza and pneumonia: 57,062 ***
- Nephritis, nephrotic syndrome, and nephrosis: 49,959 **
- Suicide: 44,193 ** (microbiota are closely linked to depression, which is a major cause of suicide)
There are many probiotic products on the market that promote the growth of beneficial bacteria in the gut, but few probiotic products reach the ascending colon to the rectum and are effective. There is a very close relationship between the level of ionic calcium in the gut and the bacteria that secrete SCFAs. It has been successful in increasing beneficial bacteria by reaching the ascending, transverse, descending, and rectum (sigmoid).
Possible Benefits of Faecalibacterium prausnitzii for Obesity-Associated Gut Disorders
Mapping Human Microbiome Drug Metabolism by Gut Bacteria and Their Genes
Individuals vary widely in their responses to medicinal drugs, which can be dangerous and expensive owing to treatment delays and adverse effects. Although increasing evidence implicates the gut microbiome in this variability, the molecular mechanisms involved remain largely unknown. Here we show by measuring the ability of human gut bacteria from diverse clades to metabolize orally administered drugs that many drugs are chemically modified by microorganisms. We combined high-throughput genetic analyses with mass spectrometry to systematically identify microbial gene products that metabolize drugs. These microbiome-encoded enzymes can directly and substantially affect intestinal and systemic drug metabolism in mice and can explain the drug-metabolizing activities of human gut bacteria and communities based on their genomic contents. These causal links between the gene content and metabolic activities of the microbiota connect interpersonal variability in microbiomes to interpersonal differences in drug metabolism, which has implications for medical therapy and drug development across multiple disease indications. In particular, long-term use of antibiotics, anticancer drugs, etc., has a very negative impact on the gut microbiome, leading to a loss of immunity and making recovery from disease very slow or impossible.
SAC Calcium and Biofilm of Microbiome
Dairies have used calcium to manage sewer pipes clogged due to biofilm formation of microorganisms in dairy wastewater. Biofilm formation in dairy wastewater system irrigation pipes can reduce treatment capacity, increasing maintenance and cleaning costs. Understanding the effect of different components in the wastewater on the growth and yield of bacteria present could help prevent excessive build-up of biofilms. This study investigated in aerobic and anaerobic conditions the effect of calcium, sodium, and magnesium concentrations on growth rates, yields, and saturation constants of four known biofilm-forming bacteria associated with the blockage of an irrigation system.
The ions tested (Ca++, Mg++, Na+) varied growth rates, with biofilm growth in the presence of calcium being significantly slower (P < 0.05) than planktonic growth in sodium. Along with the slower growth, the addition of Ca++ (up to 0.1 M) increased biofilm formation, while addition over 0.5 M prevented biofilm formation. Knowing the nutritional requirements of the bacteria and the effects of the ions will be useful in predicting the growth development and strategies in controlling biofilm formation in dairy wastewater.
“The effect of calcium on biofilm formation in dairy wastewater” by School of Food and Nutrition, Massey University, New Zealand, 2018. Water Practice & Technology Vol 13 No 2.
Fig. BFI mixed culture results in the presence of Ca++ (570 nm). Error bars represent 95% confidence intervals on nine measurements. Calcium is the independent variable. The addition of 0.5 M or greater completely inhibited biofilm formation.
Bacteria protect themselves from the external environment by creating a defense barrier called biofilm while performing quorum sensing. The same is true for the large intestine of the human body. When bad bacteria gather and form biofilm due to an imbalance in the diet, toxic substances are secreted, and when these act on mucosal cells, they will naturally harm intestinal health.
Many types of products are being released to balance the gut microbiome. Starting with probiotics, prebiotics, and products, recently synbiotics, postbiotics, and parabiotics have also been produced and sold. However, although some products are partially effective, the effect is still insufficient.
For this reason, if SAC calcium is coated with fiber to prevent absorption in the duodenum and small intestine and sent down to the rectum, SAC calcium contained in Bio-Sacal reaches the large intestine and rectum and supplies ionic calcium to beneficial bacteria (Akkermansia muciniphila, Bifidobacteria, Christensenellaceae, Faecalibacterium, etc.) that secrete SCFA in the intestine to activate their metabolism, thereby increasing the abundance of beneficial bacteria and eliminating harmful bacteria.
Through this SCFA (short chain fatty acid), it not only normalizes the immune function but also relatively reduces the secretion of harmful substances, strengthens the intestinal mucosa and intestinal muscles, eliminates constipation, and greatly helps colon health. The results of this study led to the launch of Bio-sacal, a prebiotic, in Feb. 2023.
Bio-sacal is a prebiotic that supplies calcium ions to all parts of the intestines by coating calcium with fiber through a special process so that it reaches the rectum, thereby maintaining the balance of intestinal microflora and increasing the production of short-chain fatty acids by bacteria such as Akkermansia, thereby enhancing immunity.
Case Study
Age 60 Male, NY, USA 2021 with Rectal Cancer Stage 3
Diagnosis: Rectal Cancer Stage 3 was diagnosed in Feb. 2021.The patient had a colonoscopy (9/15/22) showing an ulcerated and fungating mass 8 cm from the anus consistent with moderately differentiated adenocarcinoma, MMR intact.
Pre/concurrent SAC Treatments: Radiation ended on Dec. 2, 2022
Marah-Cel + SAC-SG started on Dec. 14, 2022
1st round of chemo started on Dec. 16, 2022
2nd round of chemo started on Jan. 6, 2023
4th round of chemo ended early March 2023
3D-SAC Therapy for Cancer
SAC-SG + Marah-Cel + Bio-Sacal was administered 3x a day since Dec. 14, 2022.
The first two rounds of chemo posed no difficulty.
The 3rd and 4th rounds of chemo posed slight difficulty.
On February 10,2023, three CT scans were performed, and the results showed that the affected rectum was treated well.
On March 1, the surgeon in charge of surgery performed a precision endoscopy and found no visible tumor on the site. The opinions of the doctors were divided on whether the surgery should still be performed or not.
He took an MRI on Mar. 14, 2023, to make the final decision. All cancer disappeared, and the surgery and any further chemotherapy were finally canceled on March 17, 2023. The patient is scheduled for check-ups every 3-6 months.
