Breast cancer remains one of the most common malignancies worldwide, and resistance to conventional therapies continues to present significant clinical challenges. As interest grows in targeting cancer metabolism and cellular signaling pathways, researchers are exploring novel approaches that may influence cancer cell survival.

This study evaluated the effects of two anti-orbital ionic calcium formulations, AIC-FA and AIC-SG, in MCF-7 estrogen receptor-positive breast cancer cells. The research investigated their impact on cell viability and cell death profiles under controlled laboratory conditions.

Results demonstrated that both compounds significantly reduced breast cancer cell viability over time. Flow cytometry analysis also revealed distinct patterns of Annexin V/PI-defined cell death, suggesting that the two formulations may influence cellular responses through different biological mechanisms.

These findings provide preclinical evidence supporting further investigation of anti-orbital ionic calcium compounds and their biological activity in cancer research models.